Product Information
|
Protein full name |
Cyclin-dependent kinase inhibitor 1 |
|
Synonyms |
CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, p21, cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase inhibitor 1A |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse p21 |
|
Uniprot ID |
P39689 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. / Observed MW. |
19 kDa / 20 kDa |
Applications
|
WB |
Human, Mouse |
1: 300-1: 600 |
293T, MCF7, T47D, NIH3T3 |
Background
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.
Images
|
|
Western blot analysis of P21 (GB115313) at dilution of 1:300 |
|
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Western blot analysis of P21 (GB115313) at dilution of 1:300 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
