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Anti-Caspase-3 Rabbit pAb
商品編號：GB115600
| Protein full name | Caspase-3 |
| Synonyms | Casp3, CASP3, CPP32, CPP32B, SCA-1, Caspase 3 |
| Immunogen | Recombinant protein corresponding to Human Caspase-3 |
| Uniprot ID | P42574 |
| Isotype | lgG |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm |
| Predicted MW. / Observed MW. | 32 kDa / 17/35 kDa |
Applications
| WB | Human | 1: 500-1: 1000 | Jurkat, THP-1, 293T |
| IHC/IF | Human | 1: 700-1: 2100 | tonsil, colon, T-cell lymphoma |
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Images
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Western blot analysis of Caspase-3 (GB115600) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded human colon using Caspase-3 (GB115600) at dilution of 1:700 |
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Immunohistochemistry analysis of paraffin embedded human T-cell lymphoma using Caspase-3 (GB115600) at dilution of 1:700 |
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Immunohistochemistry analysis of paraffin embedded human tonsil using Caspase-3 (GB115600) at dilution of 1:700 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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