IMDM，全稱Iscove's Modified Dulbecco Medium，即Iscove改良的DMEM培養基。最初設計用於培養紅血球前驅細胞和巨噬細胞。在DMEM基礎上，IMDM培養基添加了微量元素硒、額外的氨基酸和維生素，以硝酸鉀代替硝酸鐵，營養豐富，非常適合快速增殖、高密度細胞培養，例如小鼠B淋巴細胞、LPS刺激的B細胞、骨髓造血細胞、T淋巴細胞及各種雜交瘤細胞。另外，IMDM還可以作為一些獨特的無血清培養基的基礎液。
本產品含有多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含L-丙氨酰-L-谷氨酰胺，含丙酮酸鈉，並添加酚紅指示劑，含HEPES緩衝系統。具體配方請查詢官網。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
Glycine |
30.0 |
0.40 |
CalciumChloride (CaCl2)(anhyd.) |
165.0 |
1.4865 |
|
|
L-Alanine |
25.0 |
0.2809 |
Magnesium Sulfate (MgSO4) (anhyd.) |
97.67 |
0.8139 |
|
|
L-Arginine hydrochloride |
84.0 |
0.3981 |
Potassium Chloride (KCl) |
330.0 |
4.40 |
|
|
L-Asparagine |
25.0 |
0.1894 |
Potassium Nitrate (KNO3) |
0.076 |
0.0007520 |
|
|
L-Aspartic Acid |
30.0 |
0.2256 |
Sodium Bicarbonate (NaHCO3) |
3024.0 |
36.0 |
|
|
L-Cystine 2HCl |
91.4 |
0.2920 |
Sodium Chloride (NaCl) |
4505.0 |
77.67 |
|
|
L-Glutamic Acid |
75.0 |
0.5102 |
Sodium Phosphate monobasic (NaH2PO4) (anhyd.) |
108.7 |
0.9058 |
|
|
L-Alanyl-L-Glutamine |
868.9 |
4.00 |
Sodium selenite (Na2SeO3) |
0.0170 |
0.0000983 |
|
|
L-Histidine hydrochloride-H2O |
42.0 |
0.20 |
Other components |
|||
|
L-Isoleucine |
105.0 |
0.8015 |
D-Glucose (Dextrose) |
4500.0 |
25.0 |
|
|
L-Leucine |
105.0 |
0.8015 |
HEPES |
5958.0 |
25.03 |
|
|
L-Lysine hydrochloride |
146.0 |
0.7978 |
Phenol red |
14.107 |
0.0399 |
|
|
L-Methionine |
30.0 |
0.2013 |
Sodium pyruvate |
110.000 |
1.0 |
|
|
L-Phenylalanine |
66.0 |
0.40 |
||||
|
L-Proline |
40.0 |
0.3478 |
||||
|
L-Serine |
42.0 |
0.40 |
||||
|
L-Threonine |
95.0 |
0.7983 |
||||
|
L-Tryptophan |
16.0 |
0.0784 |
||||
|
L-Tyrosine Disodium Salt |
104.0 |
0.4622 |
||||
|
L-Valine |
94.0 |
0.8034 |
||||
|
Vitamins |
||||||
|
Biotin |
0.0130 |
0.00005330 |
||||
|
Choline chloride |
4.0 |
0.02857 |
||||
|
D-Calcium pantothenate |
4.0 |
0.00839 |
||||
|
Folic Acid |
4.0 |
0.009070 |
||||
|
Niacinamide |
4.0 |
0.032787 |
||||
|
Pyridoxine hydrochloride |
4.0 |
0.019608 |
||||
|
Riboflavin |
0.40 |
0.001064 |
||||
|
Thiamine hydrochloride |
4.0 |
0.01187 |
||||
|
Vitamin B12 |
0.0130 |
0.00000959 |
||||
|
i-Inositol |
7.2 |
0.040 |
||||
