M199全稱為Medium 199，即培養基199，最初設計用於雞胚成纖維細胞的培養，目前廣泛應用於各種動物細胞的培養，包括非哺乳動物細胞。與其他基礎培養基相比，M199含有一些獨特的成分，包括腺嘌呤、腺苷、次黃嘌呤、胸腺嘧啶及其他維生素，可以採用不同平衡鹽成分，例如Earle's鹽適用於CO 2環境，而Hank's鹽適用於非CO 2環境。 M199特別適用於非轉化細胞的培養，也常用於病毒學、大鼠胰臟上皮細胞及小鼠水晶體組織的培養。
本產品含有多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質、脂類或生長因子，需依細胞需求添加血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含Earle ' s平衡鹽體系，含1000 mg/L D-葡萄糖，添加L-丙氨酰-L-谷氨酰胺。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
Glycine |
50.0 |
0.6667 |
Calcium Chloride (CaCl2)(anhyd.) |
200.0 |
1.802 |
|
|
L-Alanine |
25.0 |
0.2809 |
Ferric Nitrate (Fe(NO3)3·9H2O) |
0.7 |
0.001733 |
|
|
L-Argininehydrochloride |
70.0 |
0.3318 |
MagnesiumSulfate (MgSO4) (anhyd.) |
97.7 |
0.8139 |
|
|
L-Asparagine acid |
30.0 |
0.2256 |
Potassium Chloride (KCl) |
400.0 |
5.333 |
|
|
L-Cysteine hydrochloride·H2O |
0.10 |
0.0006 |
SodiumBicarbonate(NaHCO3) |
2200.0 |
26.190 |
|
|
L-Cystine 2HCl |
33.9 |
0.1083 |
Sodium chloride (NaCl) |
6800.0 |
117.24 |
|
|
L-Glutamic acid |
75.0 |
0.5102 |
Sodium dihydrogen phosphate(NaH2PO4)(anhyd.) |
121.7 |
1.014 |
|
|
L-Alanyl-L-Glutamine |
148.8 |
0.6849 |
Other components |
|||
|
L-Histidine hydrochloride·H2O |
21.9 |
0.1042 |
D-Glucose (Dextrose) |
1000.0 |
5.556 |
|
|
L-Hydroxyproline |
10.0 |
0.0763 |
Phenol Red |
18.83 |
0.053135 |
|
|
L-Isoleucine |
40.0 |
0.3053 |
Cholesterol |
0.20 |
0.00051680 |
|
|
L-Leucine |
60.0 |
0.4580 |
Glutathione (reduced) |
0.05 |
0.00016287 |
|
|
L-Lysine hydrochloride |
70.0 |
0.3825 |
Sodium Acetate |
50.0 |
0.60976 |
|
|
L-Methionine |
15.0 |
0.1007 |
Tween 80 |
20 |
- |
|
|
L-Phenylalanine |
25.0 |
0.1515 |
Hypoxanthine Na |
0.40 |
0.0029412 |
|
|
L-Proline |
40.0 |
0.3478 |
2-deoxy-D-ribose |
0.50 |
0.003731 |
|
|
L-Serine |
25.0 |
0.2381 |
Adenine sulfate |
5.772 |
0.024752 |
|
|
L-Threonine |
30.0 |
0.2521 |
Adenosine 5’-phosphate |
0.211 |
0.0005764 |
|
|
L-Tryptophan |
10.0 |
0.0490 |
Adenosine 5’-triphosphate |
0.911 |
0.0016529 |
|
|
L-Tyrosine disodium salt dihydrate |
58.0 |
0.2222 |
Guanine hydrochloride |
0.30 |
0.00159574 |
|
|
L-Valine |
25.0 |
0.2137 |
Ribose |
0.50 |
0.0033333 |
|
|
Vitamins |
Thymine |
0.30 |
0.0023810 |
|||
|
Ascorbic Acid |
0.050 |
0.0002841 |
Uracil |
0.30 |
0.002679 |
|
|
Biotin |
0.010 |
0.0000410 |
Xanthine-Na |
0.34 |
0.002237 |
|
|
Choline chloride |
0.50 |
0.003571 |
||||
|
D-Calcium pantothenate |
0.010 |
0.0000210 |
||||
|
Folic Acid |
0.010 |
0.0000227 |
||||
|
Menadione (Vitamin K3) |
0.01606 |
0.00005814 |
||||
|
Niacinamide |
0.0250 |
0.0002049 |
||||
|
Nicotinic Acid (Niacin) |
0.0250 |
0.0002033 |
||||
|
Para-Aminobenzoic Acid |
0.0500 |
0.0003650 |
||||
|
Pyridoxal hydrochloride |
0.0250 |
0.0001225 |
||||
|
Pyridoxine hydrochloride |
0.0250 |
0.0001214 |
||||
|
Riboflavin |
0.0100 |
0.00002660 |
||||
|
Thiamine hydrochloride |
0.0100 |
0.00002967 |
||||
|
VitaminA (acetate) |
0.100 |
0.00030488 |
||||
|
Vitamin D2 (Calciferol) |
0.100 |
0.00025189 |
||||
|
Alpha Tocopherol phos. Na salt |
0.010 |
0.0000180 |
||||
|
I-lnositol |
0.050 |
0.0002778 |
||||
