McCoy's 5A, 含GlutaPlus, 含HEPES培養基是一種通用培養基，最初由Thomas McCoy等人設計專門用於Novikoff Hepatoma細胞的培養基。與其他培養基相比，McCoy's 5A modified含有高濃度的D-葡萄糖、細菌蛋白腖及還原型穀胱甘肽等成分。 McCoy's 5A modified適用於多種類型的原代細胞的培養，例如來自骨髓、皮膚、腎、脾、肺、大鼠胚胎、網膜等組織的細胞。此外，也用於組織切片培養、細胞建系，以及一些淋巴球和較難培養的細胞。
本產品含多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加5-10%血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含16.67 mM D-葡萄糖，1.5 mM L-丙氨酰-L-谷氨酰胺，含25 mM HEPES緩衝體系，含酚紅指示劑，不含丙酮酸鈉。
HEPES是一種常用生物緩衝劑，能使培養基較長時間維持恆定的pH範圍，有效防止培養液pH波動較大對細胞生長產生不利的影響。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Thiamine hydrochloride |
0.20 |
0.000593472 |
|||
|
Glycine |
7.5 |
0.1000 |
Vitamin B12 |
2.00 |
0.001476015 |
|
|
L-Alanine |
13.9 |
0.156 |
i-Inositol |
36.0 |
0.2000 |
|
|
L-Arginine hydrochloride |
42.1 |
0.1995 |
Inorganic salts |
|||
|
L-Asparagine monohydrate |
51.14 |
0.3409 |
Calcium Chloride (CaCl2)(anhyd.) |
100 |
0.901 |
|
|
L-Asparagine |
19.97 |
0.1502 |
Magnesium sulfate(MgSO4)(anhyd.) |
200 |
0.813 |
|
|
L-Cysteine hydrochloride hydrate |
31.5 |
0.2603 |
Potassium Chloride (KCl) |
400 |
5.333 |
|
|
L-Glutamic acid |
22.1 |
0.1503 |
Sodium bicarbonate(NaHCO3) |
2200 |
26.190 |
|
|
L-Alanyl-L-Glutamine |
326.1 |
1.5014 |
Sodium chloride (NaCl) |
5100 |
87.931 |
|
|
L-Histidine hydrochloride monohydrate |
20.96 |
0.0998 |
Sodium Phosphate monobasic(NaH2PO4)(anhyd.) |
504 |
4.203 |
|
|
L-Isoleucine |
19.7 |
0.1504 |
Other components |
|||
|
L-Leucine |
39.36 |
0.3005 |
Peptone |
600 |
Infinity |
|
|
L-Lysinehydrochloride |
39.36 |
0.3005 |
D-Glucose (Dextrose) |
3000 |
16.67 |
|
|
L-Lysine Hydrochloride |
36.5 |
0.1995 |
Reduced glutathione |
0.5 |
0.0016 |
|
|
L-Methionine |
14.9 |
0.1000 |
Phenol Red |
9.4 |
0.0266 |
|
|
L-Phenylalanine |
16.5 |
0.1000 |
HEPES |
5958 |
25 |
|
|
L-Proline |
17.3 |
0.1504 |
||||
|
L-Serine |
26.3 |
0.2505 |
||||
|
L-Threonine |
17.9 |
0.1504 |
||||
|
L-Tryptophan |
3.06 |
0.0152 |
||||
|
L-Tyrosine disodium salt |
26.1 |
0.1000 |
||||
|
L-Valine |
17.6 |
0.1504 |
||||
|
Vitamins |
||||||
|
L-Ascorbic acid |
0.50 |
0.002840909 |
||||
|
Biotin |
0.20 |
0.000819672 |
||||
|
Choline chloride |
5.00 |
0.035714287 |
||||
|
D-Calcium pantothenate |
0.20 |
0.000419287 |
||||
|
Folic Acid |
10.00 |
0.022675738 |
||||
|
Niacinamide |
0.50 |
0.00409836 |
||||
|
Nicotinic acid |
0.50 |
0.004065041 |
||||
|
Para-Aminobenzoic Acid |
1.00 |
0.00729927 |
||||
|
Pyridoxal hydrochloride |
0.50 |
0.002450981 |
||||
|
Pyridoxine hydrochloride |
0.50 |
0.002427185 |
||||
|
Riboflavin |
0.20 |
0.000531915 |
||||
