DMEM/F-12培養基是將DMEM與Ham's F-12培養基以1:1比例混合改良而來的。改良所得的DMEM/F-12培養基，營養成分較為豐富，含有更多種微量元素，廣泛應用於多種哺乳類細胞的培養，也適用於低血清含量下哺乳動物細胞的培養以及克隆密度培養。 DMEM/F-12也被用作開發無血清培養基的基礎培養基。
本產品含多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加5-10%血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含17.5 mM D-葡萄糖，0.5 mM丙酮酸鈉，含酚紅指示劑，不含L-麩醯胺酸，不含HEPES緩衝系統。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
Glycine |
18.75 |
0.250 |
Calcium Chloride (CaCl2)(anhyd.) |
116.6 |
1.050 |
|
|
L-Alanine |
4.45 |
0.050 |
Cupric Sulfate (CuSO4·5H2O) |
0.0013 |
0.0000052 |
|
|
L-Arginine monohydrochloride |
147.5 |
0.699 |
Ferric Nitrate (Fe(NO3)3·9H2O) |
0.05 |
0.0001238 |
|
|
L-Asparagine monohydrate |
7.5 |
0.050 |
Ferrous Sulfate (FeSO4·7H2O) |
0.417 |
0.0015 |
|
|
L-Asparagine |
6.65 |
0.050 |
Magnesium chloride (MgCl2)(anhyd.) |
28.64 |
0.301 |
|
|
L-Cysteine hydrochloride hydrate |
17.56 |
0.100 |
Magnesium sulfate (MgSO4) (anhyd.) |
48.84 |
0.407 |
|
|
L-Cystine dihydrochloride |
31.29 |
0.100 |
Potassium Chloride (KCl) |
311.8 |
4.157 |
|
|
L-Glutamic acid |
7.35 |
0.050 |
Sodium bicarbonate(NaHCO3) |
2438 |
29.024 |
|
|
L-Glutamine |
– |
– |
Sodium chloride (NaCl) |
6995.5 |
120.6 |
|
|
L-Histidine hydrochloride monohydrate |
31.48 |
0.150 |
Disodium (hydrogen) phosphate(Na2HPO4)(anhyd.) |
71.02 |
0.500 |
|
|
L-Isoleucine |
54.47 |
0.416 |
Sodium dihydrogen phosphate(NaH2PO4)(anhyd.) |
54.34 |
0.453 |
|
|
L-Leucine |
59.05 |
0.451 |
Zinc Sulfate(ZnSO4·7H2O) |
0.432 |
0.0015 |
|
|
L-Lysinehydrochloride |
91.25 |
0.499 |
Other components |
|||
|
L-Methionine |
17.24 |
0.116 |
D-Glucose (Dextrose) |
3151 |
17.51 |
|
|
L-Phenylalanine |
35.48 |
0.215 |
Hypoxanthine Na |
2.39 |
0.015 |
|
|
L-Proline |
17.25 |
0.150 |
Linoleic Acid |
0.042 |
0.00015 |
|
|
L-Serine |
26.25 |
0.250 |
Lipoic acid |
0.105 |
0.00051 |
|
|
L-Threonine |
53.45 |
0.449 |
Phenol Red |
8.1 |
0.022 |
|
|
L-Tryptophan |
9.02 |
0.044 |
Putrescine dihydrochloride |
0.081 |
0.00050 |
|
|
L-Tyrosine disodium salt |
55.79 |
0.214 |
Thymidine |
0.365 |
0.00151 |
|
|
L-Valine |
52.85 |
0.452 |
Sodium pyruvate |
55 |
0.500 |
|
|
Vitamins |
HEPES |
– |
– |
|||
|
Biotin |
0.0035 |
0.0000143 |
||||
|
Choline chloride |
8.98 |
0.0641 |
||||
|
D-Calcium pantothenate |
2.24 |
0.0047 |
||||
|
Folic Acid |
2.65 |
0.0060 |
||||
|
Niacinamide |
2.02 |
0.0166 |
||||
|
Pyridoxine hydrochloride |
2.013 |
0.0098 |
||||
|
Riboflavin |
0.219 |
0.0006 |
||||
|
Thiamine hydrochloride |
2.17 |
0.0064 |
||||
|
Vitamin B12 |
0.68 |
0.0005 |
||||
|
i-Inositol |
12.6 |
0.07 |
||||
