Ham's F-12K培養基是Ham's F-12營養混合物的Kaighn's改良型，最初是為了在低血清條件下培養原代人類肝細胞及分化的大鼠、雞的細胞而設計。 Ham's F-12K在原來Ham's F-12的基礎上提高了氨基酸和丙酮酸的濃度，降低了葡萄糖的含量，並修改了無機鹽的成分及含量。與其他培養基相比，Ham's F-12K含有許多獨特的成分，例如腐胺、胸腺嘧啶、次黃嘌呤、鋅等，此外丙酮酸鈉的含量也較高。
本產品含有多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含7.0 mM D-葡萄糖，不含L-麩醯胺酸，添加2.0 mM丙酮酸鈉，含酚紅指示劑，不含HEPES緩衝系統。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
Glycine |
15 |
0.2 |
Calcium Chloride (CaCl2)(anhyd.) |
102 |
0.9189 |
|
|
L-Alanine |
18 |
0.2022 |
Cupric Sulfate (CuSO4·5H2O) |
0.002 |
0.0000080 |
|
|
L-Arginine monohydrochloride |
422 |
2.0 |
Ferrous Sulfate (FeSO4·7H2O) |
0.8 |
0.002878 |
|
|
L-Asparagine monohydrate |
30 |
0.2 |
Magnesium chloride (MgCl2)(anhyd.) |
49.7 |
0.5231 |
|
|
L-Asparagine |
26.6 |
0.2 |
Magnesium sulfate(MgSO4)(anhyd.) |
192 |
1.600 |
|
|
L-Cysteine hydrochloride hydrate |
70 |
0.3977 |
Potassium Chloride (KCl) |
285 |
3.800 |
|
|
L-Glutamic acid |
29 |
0.1973 |
Sodium bicarbonate(NaHCO3) |
2500 |
29.76 |
|
|
L-Glutamine |
– |
- |
Sodium chloride (NaCl) |
7530 |
129.9 |
|
|
L-Histidine hydrochloride monohydrate |
45.8 |
0.2181 |
Sodium Phosphate dibasic (Na2HPO4) (anhyd.) |
115.5 |
0.813 |
|
|
L-Isoleucine |
7.88 |
0.06015 |
Sodium Phosphate monobasic (NaH2PO4) (anhyd.) |
59.0 |
0.492 |
|
|
L-Leucine |
26.2 |
0.2 |
Zinc Sulfate(ZnSO4·7H2O) |
0.144 |
0.0005 |
|
|
L-Lysinehydrochloride |
73 |
0.3989 |
Other components |
|||
|
L-Methionine |
8.96 |
0.06013 |
D-Glucose (Dextrose) |
1260 |
7 |
|
|
L-Phenylalanine |
9.92 |
0.06012 |
Hypoxanthine |
4 |
0.03053 |
|
|
L-Proline |
69 |
0.6 |
Lipoic acid |
0.21 |
0.001019 |
|
|
L-Serine |
21 |
0.2 |
Phenol Red |
3 |
0.007970 |
|
|
L-Threonine |
23 |
0.1933 |
Putrescine 2HCl |
0.32 |
0.001987 |
|
|
L-Tryptophan |
4.1 |
0.02010 |
Sodium pyruvate |
220 |
2.0 |
|
|
L-Tyrosine disodium salt |
13.5 |
0.05153 |
Thymidine |
0.7 |
0.002892 |
|
|
L-Valine |
23 |
0.1966 |
||||
|
Vitamins |
||||||
|
Biotin |
0.0073 |
0.0000299 |
||||
|
Choline chloride |
14 |
0.1 |
||||
|
D-Calcium pantothenate |
0.50 |
0.001048 |
||||
|
Folic Acid |
1.30 |
0.002948 |
||||
|
Niacinamide |
0.04 |
0.000295 |
||||
|
Pyridoxine hydrochloride |
0.06 |
0.000291 |
||||
|
Riboflavin |
0.04 |
0.000098 |
||||
|
Thiamine hydrochloride |
0.30 |
0.000890 |
||||
|
Vitamin B12 |
1.40 |
0.001033 |
||||
|
i-Inositol |
18 |
0.1 |
||||
