Ham's F-12營養混合物（Ham's F-12 Nutrient Mixture）是以Ham's F-10營養混合物為基礎設計的，最初用於CHO細胞的無血清培養。 Ham's F-12常作為無血清培養的基礎培養液，在低血清含量時特別適合單細胞培養和克隆化培養，添加血清後也廣泛應用於腫瘤細胞和原代細胞的培養，如大鼠肝細胞、大鼠前列腺上皮細胞、軟骨細胞、大鼠成肌細胞、雞胚胎細胞等。此外，Ham's F-12與DMEM等體積混合後可得到營養更豐富的DMEM/F-12培養基。
本產品含有多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含10 mM D-葡萄糖，不含L-麩醯胺酸，添加1.0 mM丙酮酸鈉，含酚紅指示劑，不含HEPES緩衝系統。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
Glycine |
7.507 |
0.1 |
Potassium Chloride (KCl) |
223.6 |
2.98 |
|
|
L-Alanine |
8.90 |
0.10 |
Sodium bicarbonate(NaHCO3) |
1176 |
14 |
|
|
L-Arginine monohydrochloride |
210.66 |
1.0 |
Sodium chloride (NaCl) |
7599 |
131.02 |
|
|
L-Asparagine monohydrate |
15.02 |
0.10 |
Sodium Phosphate dibasic (Na2HPO4) (anhyd.) |
141.96 |
1.0 |
|
|
L-Asparagine |
13.31 |
0.1 |
Calcium Chloride (CaCl2)(anhyd.) |
33.22 |
0.30 |
|
|
L-Cysteine hydrochloride hydrate |
35.05 |
0.20 |
Cupric Sulfate (CuSO4·5H2O) |
0.0025 |
0.00001 |
|
|
L-Glutamic acid |
14.71 |
0.1 |
Ferrous Sulfate (FeSO4·7H2O) |
0.834 |
0.003 |
|
|
L-Glutamine |
- |
- |
Magnesium chloride (MgCl2)(anhyd.) |
57.35 |
0.60 |
|
|
L-Histidine hydrochloride monohydrate |
20.96 |
0.1 |
Zinc Sulfate(ZnSO4·7H2O) |
0.862 |
0.003 |
|
|
L-Isoleucine |
4.005 |
0.031 |
Other components |
|||
|
L-Leucine |
13.12 |
0.1 |
D-Glucose (Dextrose) |
1802.0 |
10.01 |
|
|
L-Lysinehydrochloride |
36.50 |
0.199 |
Hypoxanthine |
1.13 |
0.00319 |
|
|
L-Methionine |
4.51 |
0.03 |
Lipoic acid |
110 |
1.0 |
|
|
L-Phenylalanine |
5.01 |
0.03 |
Phenol Red |
4.083 |
0.03 |
|
|
L-Proline |
34.5 |
0.3 |
Putrescine 2HCl |
0.084 |
0.0003 |
|
|
L-Serine |
10.5 |
0.1 |
Sodium pyruvate |
0.210 |
0.00102 |
|
|
L-Threonine |
11.9 |
0.10 |
Thymidine |
0.16107 |
0.001 |
|
|
L-Tryptophan |
2.0 |
0.01 |
D-Glucose (Dextrose) |
0.701 |
0.00289 |
|
|
L-Tyrosine disodium salt |
7.8 |
0.0298 |
||||
|
L-Valine |
11.7 |
0.1 |
||||
|
Vitamins |
||||||
|
Biotin |
0.0073 |
0.0000299 |
||||
|
Choline chloride |
14 |
0.1 |
||||
|
D-Calcium pantothenate |
0.50 |
0.001048 |
||||
|
Folic Acid |
1.30 |
0.002948 |
||||
|
Niacinamide |
0.04 |
0.000295 |
||||
|
Pyridoxine hydrochloride |
0.06 |
0.000291 |
||||
|
Riboflavin |
0.04 |
0.000098 |
||||
|
Thiamine hydrochloride |
0.30 |
0.000890 |
||||
|
Vitamin B12 |
1.40 |
0.001033 |
||||
|
i-Inositol |
18 |
0.1 |
||||
