α-MEM培養基（α Minimum Essential Medium）是改良自MEM的培養基。與MEM培養基相比，α-MEM添加了丙酮酸鈉、硫酸鋅、維生素B12、生物素、抗壞血酸等成分，廣泛應用於各種哺乳動物懸浮和貼壁細胞的培養。 α-MEM分含核苷與不含核苷兩種。不含核苷和脫氧核苷的α-MEM常用作DG44和其他DHFR-缺陷型細胞的篩選培養基。
本產品含有多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含L-丙氨酰-L-谷氨酰胺，含丙酮酸鈉，添加酚紅指示劑，不含核苷及脫氧核苷，不含HEPES緩衝系統。具體配方請查詢官網。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
Glycine |
50.0 |
0.6667 |
CalciumChloride (CaCl2)(anhyd.) |
200 |
1.801802 |
|
|
L-Alanine |
25.0 |
0.2809 |
Magnesium Sulfate (MgSO4) (anhyd.) |
97.67 |
0.813917 |
|
|
L-Arginine hydrochloride |
105.0 |
0.4976 |
Potassium Chloride (KCl) |
400 |
5.333 |
|
|
L-Asparagine-H2O |
50.0 |
0.3333 |
Sodium Bicarbonate (NaHCO3) |
2200 |
26.2 |
|
|
L-Aspartic Acid |
30.0 |
0.2256 |
Sodium Chloride (NaCl) |
6800 |
117.2 |
|
|
L-Cystine 2HCl |
31.0 |
0.0990 |
Sodium Phosphate monobasic (NaH2PO4) (anhyd.) |
121.7 |
1.014 |
|
|
L-Cysteine hydrochloride-H2O |
100.0 |
0.5682 |
Other components |
|||
|
L-GlutamicAcid |
75.0 |
0.5102 |
D-Glucose (Dextrose) |
1000 |
5.6 |
|
|
L-Alanyl-L-Glutamine |
434.4 |
2.0 |
Lipoic Acid |
0.20 |
0.0009709 |
|
|
L-Histidine hydrochloride-H2O |
42 |
0.20 |
Phenol Red |
9.40 |
0.026567 |
|
|
L-Isoleucine |
52.4 |
0.40 |
Sodium pyruvate |
110 |
1.0 |
|
|
L-Leucine |
52.0 |
0.3969 |
Adenosine |
10.00 |
0.375 |
|
|
L-Lysine hydrochloride |
73.0 |
0.3989 |
Cytidine |
10.00 |
0.0412 |
|
|
L-Methionine |
15.0 |
0.1007 |
Guanosine |
10.00 |
0.0353 |
|
|
L-Phenylalanine |
32.0 |
0.1939 |
Uridine |
10.00 |
0.0410 |
|
|
L-Proline |
40.0 |
0.3478 |
2’Deoxyadenosine |
10.72 |
0.0398 |
|
|
L-Serine |
25.0 |
0.2381 |
2’Deoxycytidine HCl |
9.46 |
0.0417 |
|
|
L-Threonine |
48.0 |
0.4034 |
2’Deoxyguanosine |
10.68 |
0.0375 |
|
|
L-Tryptophan |
10.0 |
0.0490 |
Thymidine |
10.00 |
0.0413 |
|
|
L-Tyrosine Disodium Salt |
52.0 |
0.2311 |
||||
|
L-Valine |
46.0 |
0.3932 |
||||
|
Vitamins |
||||||
|
Ascorbic Acid |
50.0 |
0.2841 |
||||
|
Biotin |
0.10 |
0.000410 |
||||
|
Choline chloride |
1.0 |
0.007143 |
||||
|
D-Calcium pantothenate |
1.0 |
0.002096 |
||||
|
Folic Acid |
1.0 |
0.002268 |
||||
|
Niacinamide |
1.0 |
0.008197 |
||||
|
Pyridoxine hydrochloride |
1.0 |
0.004902 |
||||
|
Riboflavin |
0.10 |
0.000266 |
||||
|
Thiamine hydrochloride |
1.0 |
0.002967 |
||||
|
Vitamin B12 |
1.36 |
0.001004 |
||||
|
i-Inositol |
2.0 |
0.011111 |
||||
