MEM培養基（Minimum Essential Medium），即最低必需培養基，也稱為最小基本培養基、低限量Eagle培養基，是動物細胞培養中最常使用的基礎培養基之一。 MEM培養基僅含12種必需胺基酸、8種維生素及基礎無機鹽，營養成分簡單，主要用於貼壁細胞的培養，經配方修訂後也可用於其他類型細胞培養。
本產品含有多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加血清或無血清添加物使用。
本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含5.5 mM D-葡萄糖，添加酚紅指示劑，不含L-麩醯胺酸，不含丙酮酸鈉，不含HEPES緩衝系統。不含非必需胺基酸（NEAA）。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
L-Arginine hydrochloride |
126.0 |
0.5972 |
Calcium Chloride (CaCl2) (anhyd.) |
200 |
1.802 |
|
|
L-Cystine 2HCl |
31.0 |
0.09904 |
Magnesium Sulfate (MgSO4) (anhyd.) |
200 |
0.813 |
|
|
L-Glutamine |
292.0 |
2.0 |
Potassium Chloride (KCl) |
400 |
5.333 |
|
|
L-Histidine hydrochloride-H2O |
42.0 |
0.20 |
Sodium Bicarbonate (NaHCO3) |
2200 |
26.19 |
|
|
L-Isoleucine |
52.0 |
0.3969 |
Sodium Chloride (NaCl) |
6800 |
117.2 |
|
|
L-Leucine |
52.0 |
0.3969 |
Sodium Phosphate monobasic (NaH2PO4)(anhyd.) |
122 |
1.014 |
|
|
L-Lysine hydrochloride |
73.0 |
0.3989 |
Other components |
|||
|
L-Methionine |
15.0 |
0.1007 |
D-Glucose (Dextrose) |
1000 |
5.556 |
|
|
L-Phenylalanine |
32.0 |
0.1939 |
Phenol Red |
9.4 |
0.02657 |
|
|
L-Threonine |
48.0 |
0.4034 |
HEPES |
– |
– |
|
|
L-Tryptophan |
10.0 |
0.04902 |
||||
|
L-Tyrosine disodium salt dihydrate |
52.0 |
0.1992 |
||||
|
L-Valine |
46.0 |
0.3932 |
||||
|
Vitamins |
||||||
|
Choline chloride |
1.0 |
0.007143 |
||||
|
D-Calcium pantothenate |
1.0 |
0.002096 |
||||
|
Folic Acid |
1.0 |
0.002268 |
||||
|
Niacinamide |
1.0 |
0.008197 |
||||
|
Pyridoxine hydrochloride |
1.0 |
0.004902 |
||||
|
Riboflavin |
0.1 |
0.0002660 |
||||
|
Thiamine hydrochloride |
1.0 |
0.002967 |
||||
|
i-Inositol |
2.0 |
0.01111 |
||||
