DMEM，即Dulbecco's Modified Eagle Medium，Dulbecco's改良培養基，是在MEM培養基的基礎上改良而來，根據葡萄糖含量不同分低糖和高糖兩種。 DMEM低糖培養基適合代謝作用慢、依賴性貼壁細胞的培養。
本產品含多類細胞培養所需的胺基酸、維生素、無機鹽等成分，不含蛋白質或生長因子，需依細胞種類添加5-10%血清或無血清添加物使用。
本產品經本產品經0.1 μm濾膜過濾除菌，pH 7.0-7.4，含5.5 mM D-葡萄糖及1.0 mM丙酮酸鈉，不含L-麩醯胺酸，不含HEPES緩衝體系，不含酚紅指示劑。
|
Components |
Concentration |
Components |
Concentration |
|||
|
mg/L |
mM |
mg/L |
mM |
|||
|
Amino acids |
Inorganic salts |
|||||
|
Glycine |
30 |
0.4 |
Calcium Chloride (CaCl2)(anhyd.) |
200 |
1.80 |
|
|
L-Arginine hydrochloride |
84 |
0.4 |
Ferric nitrate nonahydrate ( Fe(NO3)3•9H2O) |
0.1 |
0.0002 |
|
|
L-Cystine dihydrochloride |
62.6 |
0.2 |
Magnesium Sulfate (MgSO4) (anhyd.) |
97.67 |
0.80 |
|
|
L-Histidine hydrochloride-H2O |
42 |
0.2 |
Potassium Chloride (KCl) |
400 |
5.36 |
|
|
L-Isoleucine |
105 |
0.8 |
Sodium Bicarbonate (NaHCO3) |
3700 |
44.04 |
|
|
L-Leucine |
105 |
0.8 |
Sodium Chloride (NaCl) |
6400 |
109.50 |
|
|
L-Lysine hydrochloride |
146 |
0.8 |
Sodium Phosphate monobasic (NaH2PO4)(anhyd.) |
108.7 |
0.906 |
|
|
L-Methionine |
30 |
0.2 |
Other components |
|||
|
L-Phenylalanine |
66 |
0.4 |
D-Glucose (Dextrose) |
1000 |
5.55 |
|
|
L-Serine |
42 |
0.4 |
Sodium pyruvate |
110 |
1.0 |
|
|
L-Threonine |
95 |
0.8 |
HEPES |
– |
– |
|
|
L-Tryptophan |
16 |
0.08 |
Phenol Red |
– |
– |
|
|
L-Tyrosine disodium salt dihydrate |
103.79 |
0.4 |
||||
|
L-Valine |
94 |
0.8 |
||||
|
L-Glutamine |
- |
- |
||||
|
L-Alanyl-L-Glutamine |
- |
- |
||||
|
Vitamins |
||||||
|
Choline chloride |
4.0 |
0.0286 |
||||
|
D-Calcium pantothenate |
4.0 |
0.0084 |
||||
|
Folic Acid |
4.0 |
0.0091 |
||||
|
Niacinamide |
4.0 |
0.0328 |
||||
|
Pyridoxine hydrochloride |
4.0 |
0.0195 |
||||
|
Riboflavin |
0.4 |
0.0011 |
||||
|
Thiamine hydrochloride |
4.0 |
0.0119 |
||||
|
i-Inositol |
7.2 |
0.0389 |
||||
